Russian botanist Tswett invented chromatography as a separation technique. He describes in detail the separation of pigments, the coloured substances by filtration through column, followed by developments with pure solvents. The aim of this research works is to develop a novel, accurate, precise,cost – effective analytical method by using RP-HPLC method , for the selected immunosuppressive agent (voclosporin). Voclosporin is a calcineurin inhibitor for the treatment of lupus nephritis (LN) in patients diagnosed with systemic lupus erythematosus (SLE). Based up on the solubility of the drugs, diluent was selected, Acetonitrile and Water taken in the ratio of 50:50. A Standard solution of Voclosporin working standard was prepared as per procedure and was injected five times into the HPLC system. Chromatographic conditions used are stationary phase Kromasil (250mm*4.6mm 5?), Mobile phase0.01N Kh2po4 Buffer: Acetonitrilein the ratio of 60:40 and flow ratewas maintained at 1ml/min, detection wave length was282nm, column temperature was set to30oCand diluent was mobile phaseConditions were finalized as optimized method

In the present work, floating microspheres of Terfenadine using Chitosan, Eudragt S 100, Eudrgit L100 as copolymers were formulated to deliver Terfenadine via oral route. The results of this investigation indicate that ionic crosslinking technique Ionotropic gelation method can be successfully employed to fabricate Terfenadine microspheres. The technique provides characteristic advantage over conventional microsphere method, which involves an “all-aqueous” system, avoids residual solvents in microspheres. Other methods utilize larger volume of organic solvents, which are costly and hazardous because of the possible explosion, air pollution, toxicity and difficult to remove traces of organic solvent completely. Micromeritic studies revealed that the mean particle size of the prepared microspheres was in the size range of 563-883µm and are suitable for bio adhesive microspheres for oral administration. Increase in the polymer concentration led to increase in % Yield, % Drug entrapment efficiency, Particle size, % swelling and % Mucoadhesion. Based on the in-vitro dissolution studies T4 formulation prepared with Chitosan 250 mg was considered as the best formulation since it has shown the maximum drug release of 97.15% in 12hrs compared to all other formulations